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Immunostaining involves the use of specific antibodies to detect the presence of pluripotency markers, such as Oct4, Nanog, SSEA-4, and Tra-160, in iPSC [79]

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39,40 In addition to delivering Cu to the secretory compartment and cytosolic proteins, Cu is targeted to the mitochondria, where cytochrome oxidase (COX) uses Cu for oxidative phosphorylation and mitochondrial function