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N., Uramoto, G

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Following differential centrifugation, the P2 microsomal membrane fraction was resuspended in cold buffer (50 mm Tris-HCl, 150 mm NaCl, 12.5 mm MgCl2, 0.2% BSA, and 10% glycerol, pH 7.4), supplemented with protease inhibitor cocktail and PhosSTOP phosphatase inhibitor cocktail, aliquoted, and stored at 80 C until use

[DOI] [PubMed] [Google Scholar] 170.Koopman N., Katsavelis D., Hove A.S.T., Brul S., Jonge W.J., Seppen J