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However, owing to the largely conserved reaction mechanism of GS and by extension, largely conserved active site residues (Figure 1), many of these inhibitors act broadly on GS homologues or isoforms suggesting that future development of nonorthosteric inhibitors may be fruitful and may be inspired by natural mechanisms that regulate GS activity

FIGURE 3 4 Strategies to further improve nicotinic acid production 4.1 Nitrilase engineering for nicotinic acid production Many attempts have been made to produce nicotinic acid using enzyme/genetic engineering