glutathione peroxidase and alcoholism (GPX) specific activity in the serum of control Glutathione Participation in the Prevention

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The researchers on this paper speculated that thymoquinone, or TQ, the primary active constituent of [BSO], may have been primarily responsible for the effect because TQ protects cells against oxidative damage induced by a variety of free radical-generating pathologies, and it also stimulates the production of acetylcholine, which causes the release of melanin and darkening of the skin through stimulation of cholinergic receptors

The study used 36 male Sprague-Dawley rat pup, exposed to 250 mg/kg PPA to induce ASD-lke features, and pre-treated for 27 days with either yogurt, artichoke extract (400 mg/kg), luteolin (50 mg/kg) or Lacticaseibacillus rhamnosus GG (0.2 mL/day)