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Briefly, 5.0 10 5 cells were transfected with 1.5 g of DNA plasmid and 3 L of Lipofectamine 2000 in a 12-well plate setting according to the manufacturers instructions

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The proposed mechanism is that in the absence of efficient GST-mediated conjugation, SFN is cleared more slowly, allowing it to circulate in its active form for a longer duration and potentially exert stronger biological effects (57)

These DAMs showing the highest variations, representing the main downregulated or upregulated metabolites across all samples, mainly belonged to the lipids, amino acids, peptides, analogs, carbohydrates, carbonyl compounds, phenols or flavonoids, indicating their significance as the characteristic metabolites