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The culture medium was supplemented with 10 % (v/v) fetal bovine serum (FBS), penicillin (100 U/ml), and streptomycin (100 g/ml)

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Meanwhile, HF (high fat) diet elevated MRS in the mouse colon, and MRS catalyzed the production of Hcy (homocysteine) (Pera-Kajn et al., 2007) and HTL (homocysteine thiolactone) (Wang et al., 2018)