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Cells were dehydrated with an ethanol series (20%, 50%, 70%, 90% and 100%) on ice for 1 min each and then 100% dry ethanol at room temperature three times for 1 min each, infiltrated with 50:50 dry ethanol:Durcupan ACM resin (Sigma-Aldrich, 44610) for 30 min and then four changes of Durcupan at 1 h each and finally embedded in a vacuum oven at 60 C for 72 h

When a supplier starts with WFI-quality water rather than ordinary purified or distilled water, the entire batch carries a higher baseline of safety before the benzyl alcohol and sterile filtration steps are ever applied

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Although we have confirmed the accumulation of Lf-LPs by fluorescently labelled LPs, we did not directly evaluate the concentration of ASX in brain