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For the analysis of staged adults, cultures ranging from 25,000-75,000 synchronized L1 larvae obtained from alkaline bleach treatment were added to 50- or 125 mL Erlenmeyer flasks containing S-complete medium such that the density of worms was maintained at approximately 3000 nematodes / mL, and kanamycin added at 35 g/mL to prevent contamination

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11 Pregnancy and Live Birth Rates: Limited Evidence We want to be honest with you here: when it comes to the outcome that matters most, actually getting pregnant and bringing home a baby, the evidence is still limited and inconclusive