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Description

Screening of missing proteins in the human liver proteome by improved MRM-approach-based targeted proteomics

While tightly regulated MLCK and ROCK signalling supports normal epithelial transport and barrier modulation, excessive or sustained activation of these pathways contributes to pathological intestinal barrier dysfunction observed in inflammatory and infectious conditions (Xiong et al., 2026)

Incell NMR spectroscopy further reveals that a range of WTL mutants (A4V, I35T, G37R, G93A, I113T) exist predominantly as unstructured apoproteins unable to bind zinc when overexpressed in human HEK293T cells, or when expressed and purified from E