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This process originates from the conversion of glutamine into glutamate through the catalysis of GLS, followed by the conversion of glutamate into -ketoglutarate (-KG) through catalysis by glutamine dehydrogenase (GDH) or transaminase (Fig

The following antibodies were used for whole mount immunolabeling of the RPE/choroid tissue: (i) antiCD31 antibodies (rat antimouse CD31 (MEC13.3, BD PharMingen)) to detect blood vessels (Alexa Fluor488 secondary antibody), (ii) antismooth muscle actin (SMA) antibodies [mouse monoclonal SMACy3 conjugate (clone 1A4, Sigma)], and (iii) Alexa647conjugated rat antimouse F4/80 (clone BM8, Biolegend) to detect macrophages

Vegans and vegetarians may not get enough B12 from their diet