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The CD8 + T cells were cultured and expanded in T cell media, which comprised RPMI 1640 supplemented with 10% fetal bovine serum, 20 mM HEPES, 1 mM sodium pyruvate, 0.05 mM 2-mercaptoethanol, 2 mM L-glutamine, 100 U/mL streptomycin, and 100 mg/mL penicillin, along with 20 ng/mL of recombinant mouse IL-2 (BioLegend, Cat# 575406)