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10.1016/S0021-9258(18)37983-3 J

GSH Quantification Glutathione and GSSG were measured in lysates from cells in 6-well plates as previously described ( ROS Quantification by Spin-Trapping and EPR Spectroscopy Cells (5 10 6 /0.1 ml) were stimulated for 2 h with LPS, then incubated with 50 mM 5-tert-butoxycarbonyl-5-methyl-1-pyrroline-N-oxide (BMPO), synthetized as described previously ( Cell Viability Assay Cells were seeded in 96-well plates at 25,000/well in complete medium

Similar trends in terms of metabolite changes were observed between all five tested UPEC strains (Tables S1 and S2), showing an accumulation of cadaverine and TMA, whereas a maximal amount of putrescine was produced after 3 h and diminished thereafter

UPS, Ubiquitin-Proteasome System