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IF and IHC staining of tissue sections Tumour tissue sections were deparaffinized in Histo-Clear (Scientific Laboratory Supplies) for 5 min, rehydrated through a series of decreasing ethanol concentrations, followed by a final 5 min wash in distilled water (dH 2 O)

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A review in Aesthetic Surgery Journal confirming that calcium hydroxylapatite biostimulators produce progressive collagen induction over 12 months, making them superior to hyaluronic acid for patients with ongoing structural changes supports the clinical logic of using biostimulators early and adaptively in weight loss patients