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Nucleases that induce double-stranded DNA breaks, such as ZFN and TALEN, can used to engineer knockin mice

doi: 10.1021/acsnano.6b08731 189 ShiYGongMDengZLiuHChangYYangZet al

Ultra-Performance Liquid Chromatography (UPLC) Analysis of Glucosinolates Total GSLs were extracted from 150 mg leaves of 3-week-old seedlings or 20 mg mature seeds according to previously reported protocols ( g for 10 min, collect supernatant and repeat the extraction one more time