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Utilizing the SYBR Green master mix (G BIOSCIENCES, Product code-786-5062) for the gene expression analysis, the following steps were performed at 10 s at 95C for denaturation, 1 min at 58 C for annealing the DNA templates, and 1 min/kb at 72 C for 40 cycles for the extension of the DNA templates

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Nuclei were isolated, and DNA from 150-g nuclear samples was sheared into fragments of 200 ~ 1000 bp by sonication