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Flow cytometry Apoptosis was detected using a FITC Annexin V Apoptosis Detection Kit (BD, La Jolla, CA, USA)

In a controlled, randomized crossover trial by Cox et al., trained endurance athletes ( n = 39) ingested a ketone ester drink at a dose of 573 mg/kg body weight, corresponding to approximately 500 mg/kg -hydroxybutyrate equivalent, which elevated circulating D-HB concentrations to ~ 3 mM within 3060 min