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In neuromuscular weakness, inspiratory muscle fatigue prevents full lung expansion, while expiratory muscle weakness prevents maximal exhalation, uniquely resulting in an elevated residual volume

Multidrug resistance: an emerging crisis

Several required mechanical ventilation

Since the refolding procedure that is required to obtain disulfide containing proteins in their native state can lead to loss of the thioester functionality, NCL is typically performed directly on the immobilized fusion protein to yield a stable amide bond, followed by refolding in redox buffer systems [18, 19]