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Jiang, H., Wu, S.L., Karger, B.L

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Required Equipment and Materials Microplate reader (with 405 nm filter or 415 nm filter) 96-well microplate 20 l, 200 l and multi-channel pipettes Incubator (37C) 5-Sulfosalicylic acid (SSA) Precaution Use the reagents in the kit after the reagents temperatures are equilibrated to the room temperature

CSE, which simplifies the exposure process by focusing on hydrophilic constituents, does not fully represent the complex mixture of chemicals and particulate matter found in whole cigarette smoke 17,18