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The amount of DON in each sample was determined using a high-performance liquid chromatography-mass spectrometer/mass spectrometer (HPLC-MS/MS) system (a Shimadzu 30A LC system coupled to a Triple Quad 6500+, Sciex, United States)

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Add 1,000 L (1 mL) of reconstitution solvent to the 1 mg vial This yields a 1 mg/mL (1,000 g/mL) stock solution To achieve 100 g/mL working stock: Dilute 100 L of 1 mg/mL stock into 900 L of PBS or cell culture medium yields 100 g/mL in 1 mL To achieve 10 ng/mL in a 5 mL experiment: Take 0.5 L of 100 g/mL working stock + 4,999.5 L of medium Alternatively: dilute to an intermediate concentration to reduce pipetting error Use a dilution series approach (stock working stock final experimental concentration) to minimize pipetting inaccuracy at nanogram-level concentrations

Dec 2015;11(3):202-6