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In total, 1 L of each extract was injected to the respective column, employing a flow rate of 100 L/min for separation

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elegans : Caenorhabditis elegans CEPs: Cephalic sensilla neurons DAT: Dopamine transporter DHE: Dihydroethidium DOPAC: 3,4-Dihydroxyphenylacetate acid DOPAL: 3,4-Dihydroxyphenylacetaldehyde PD: Parkinsons disease ROS: Reactive oxygen species GABA: Y-aminobutyric acid GFP: Green fluorescent protein ILS: Insulin-like signaling KRS: Kufor Rakeb syndrome L-DOPA: L-3,4-dihydroxyphenylalanine MAO-B: Monoamine oxidase B MDA: Malondialdehyde mitoUPR: Mitochondrial unfolded protein response MPP + : 1-Methyl-4-phenylpyridinium MPTP: 1-Methyl-4-phenyl-1,2,3,6-tetrahydropyridine PDEs: Posterior deirid neurons SN: Substantia nigra TH: Tyrosine hydroxylase VMAT2: Vesicular monoamine transporter References Akinyemi AJ, Miah MR, Ijomone OM, Tsatsakis A, Soares FAA, Tinkov AA et al (2019) Lead (Pb) exposure induces dopaminergic neurotoxicity in Caenorhabditis elegans : involvement of the dopamine transporter

Always start with a lower dose if you're unsure