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As a control, we incubated populations of gsh1 -cells at the same starting density in fresh media without any glutathione (grey curves)

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After 48 h, biofilms were washed once with 1 PBS and treated using one of the following: 30 mM GSH or 4 g/mL amikacin individually or a combination of 30 mM GSH + 4 g/mL amikacin