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3.5 Biomass and moisture content of embryogenic callus Fresh weight measurements in V

Dosing and instructions : Phenylephrine 100-500 mcg doses suspended in 1 ml of normal saline (optimally premixed by pharmacy to minimize risks of miscalculation/overdose) Doses administered 5 minutes apart Administered intracavernosally (not subcutaneously) Administered laterally (3 or 9 oclock position) near the base of the penile shaft May be continued for up to 1 hour (see commentary above) Small needles may be used (e.g., 27G) Consider performing a penile block with local anesthetic prior to beginning In cases where the combination of phenylephrine and aspiration/irrigation are performed, aspiration should precede phenylephrine administration to permit fresh, oxygenated blood to fill the corpora and potentially improve the yield of phenylephrine administration Appendix B Sample Protocol for Aspiration and Irrigation: The following protocol is one potential example of aspiration/irrigation with instillation of phenylephrine

Prepare the 27.6% (w/v) iodixanol stock solution by mixing 27.5 vol

When you donate a pint through the American Red Cross, the draw is 470 mL in about 10 minutes