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(2) We applied cryo-EM to demonstrate that, when incubated with liposomes, Arabidopsis MVB-localized, BAR domain-containing SNX proteins generate significantly shorter tubules than mouse SNX1 in vitro

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Proteins from the gel were electroblotted onto nitrocellulose membranes (Schleicher and Schuell) at 100V for 1 h, using BioRad Mini Trans-Blot transfer cells