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Ben Greenfield's protocol suggests 150mcg administered 1 hour before bed, three times weekly

both routes require a prescription Frequency Daily or every other day for subcutaneous injection protocols

At clinical doses under supervision, the most common side effects are mild nausea in the first week (typically resolves by day 7 to 10), injection site reactions (redness, minor swelling, localized soreness lasting 24 hours), and occasional transient fatigue or mild headache in the first few days

Swim latency, swim distance, time spent in the target quadrant, and number of quadrant crossings were recorded using a computerized video tracking system [2]