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Glutathione Stransferases (GSTs): GSTs lower the pKa of the GSH thiol to catalyze conjugation to electrophiles (Phase II metabolism), and some isoforms exhibit peroxidase-like activity toward lipid hydroperoxides, thereby linking detoxification with redox control

In cultured dermal fibroblasts, assays quantify pro-collagen I/III and matrix metalloproteinases (MMP-1, MMP-9) alongside their inhibitors (TIMPs)

Another study with CuCl 2 treated Hep G2 cell line demonstrated that mitochondrial toxicity is an intermediate-level event associated with copper toxicity to Hep G2 cells while lysosomal damage is the early event and plasma damage a late feature associated with copper toxicity 34

Induction of an aging mRNA retinal pigment epithelial cell phenotype by matrix-containing advanced glycation end products in vitro