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Mutagenesis: Valproate was not mutagenic in an in vitro bacterial assay (Ames test), did not produce dominant lethal effects in mice, and did not increase chromosome aberration frequency in an in vivo cytogenetic study in rats

FIGURE 1 Cell culture and establishment of OGD/R model An embryonic cardiomyocyte cell line, H9C2, was purchased from the Cell Bank of the Chinese Academy of Sciences, Shanghai, China

3.5 In vitro assessment of cell toxicity and combined treatment The cytotoxicity of different formulations was studied using CCK-8 assay

[Google Scholar] 10.Iannuzzo G., Campanozzi A., Trevisani V., Rutigliano I., Abate V., Rendina D., De Filippo G