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coli isolates ranged from 0.625 mg/ml to 2.5 mg/ml with the most effective concentration of vitamin C against most isolates at 1.25 mg/ml, similar results were found by Mahmood and Abdullah in Egypt, who reported that MIC of vitamin C was 5 mg/ml against all coliform species [18]

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They result from a combination of factors such as decreased collagen and elastin production, loss of hydration, and cumulative damage from environmental factors like UV exposure