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Description

-glutamyl peptide synthesis by GCLC scavenges glutamate to protect against ferroptosis The tripeptide -glutamyl-2-aminobutyryl-glycine (-Glu-2AB-Gly) is known to be generated by GCLC and GSS (Huang et al., 1988

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After 5 min at 95C followed by centrifugation for 10 min at 12,000 g, 20 L of the resulting supernatant was diluted with 80 L deionized water for subsequent aldehyde determination