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SESTRIN2 and SAR1B interact with and inhibit GATOR2 in the absence of leucine, and this inhibition is lifted when leucine is present 64,84,85 (Fig

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P., Harper, J

Finally, the slides were sealed with an anti-quenching agent, and the results were observed under an Olympus BX53 fluorescence microscope (Olympus Life Science, Waltham, MA) using the CellSens Dimension software (Olympus Life Science)