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In experiments in which large amounts of acetyl-CoA were incubated with purified enzyme to test its protection against SNO-CoA-mediated inhibition, varying levels of acetyl-CoA were added back into the assay solutions after the incubation period, such that in the assay condition, the concentration of acetyl-CoA was even across samples to control for the possible effect of acetyl-CoA as a competitive inhibitor of PDHC

Discussion DKD is the most common microvascular complication of diabetes, characterized by multifactorial pathogenesis [31]

Fractions with a purity of greater than 95% were pooled and lyophilized

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