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Subsequently, the undiluted samples were analyzed on an HPLC system (1200 series, Agilent Technologies Deutschland GmbH, Bblingen, Germany) with an organic acid resin column (300 mm 8 mm) and the corresponding pre-column (40 mm 8 mm) (Chromatographie-Service GmbH, Langerwehe, Germany) heated to 60C

10,11 The ovaries are also made up of theca and granulosa cells, responsible for secreting estrogen, progesterone, androgens (male hormones), and other factors that regulate follicular activation and maturation