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Preclinical studies in in vitro models have shown that simvastatin exerted its anti-tubercular activity through cholesterol-driven autophagy, which is mediated by the adenosine monophosphate-activated protein kinase (AMPK)mammalian target of the rapamycin complex 1 (mTORC1)TFEB axis [58] (Fig

First, the periplasmic fractions were bound to HisPur Ni-NTA Resin (Thermo 88222) and then washed sequentially with TBS (20 mM Tris, 150 mM NaCl) containing 20 mM and 40 mM imidazole, followed by elution with TBS containing 500 mM imidazole

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