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Affinity purification performed by gravity using TALON resin (Clontech), anion exchange performed using HiTrapQ FF (GE Healthcare), size-exclusion chromatography performed using Superdex 200 10/30 prep grade (GE Healcare) the later two performed using KTA FPLC platform

Integrated multi-omics reveals anaplerotic rewiring in methylmalonyl-CoA mutase deficiency

Saturated fat and cardiometabolic risk factors, coronary heart disease, stroke, and diabetes: a fresh look at the evidence

The intervention was delivered as single weekly intravenous infusion, with each dose administered over 15 min in 50 mL of saline, for two consecutive 8-week periods, separated by a 1-week washout