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A narrative review of positive regulation of NLRP3 inflammasome in rheumatoid arthritis

for example, dihydroethidium (DHE) can be oxidized to ethidium (E) in addition to the diagnostic 2-hydroxyethidium (2-OH-E), so raw fluorescence tends to overcall superoxide, and imaging can add phototoxicity and dose/time artifacts unless illumination and local chemistry are tightly controlled

The VH and VL were amplified in 2 separate reactions using degenerate primers (GE Healthcare)