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ExpiCHO-S cells were transfected with pCAGGS-AIM with each mutation and cultured in ExpiCHO Expression Medium supplemented with ExpiCHOFeed and ExpiFectamine CHO Enhancer for 4 days in a 37 C incubator with a humidified atmosphere of 8% CO 2 in air on an orbital shaker

Hence, ferroptosis is likely not a major contributing factor in APAP-induced hepatotoxicity under physiological conditions

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