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Specifically, the pathologic effects of anti-GAD65Abs on GABA synthesis and release were specific to anti-GAD65Ab b78representing SPS- and CA-associated anti-GAD65Aband could not be reproduced by monoclonal anti-GAD65Ab b96.11, representing an epitope specificity associated with type 1 diabetes mellitus [80, 81, 82]

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Precipitated proteins were centrifuged 15 min at 16,000 g at 4 C and 0.6 mL cold acetone was added to the pellet