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Description

2.8 Measurement of nucleoside triphosphates Nucleotides were extracted from 4 to 5 10 6 cell pellets using perchloric acid, and the extracts were neutralized with potassium hydroxide

Moreover, this speculation was also confirmed by further EMSA experiment that SlBEL11 directly binds to the promoter of SlLCY-b2 containing TTGACTTGAC atagt GTCA element (Figure 3)