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To address this, we used a series of orthogonal approaches to test the role of NAD(P) + homeostasis in chemotherapy induced infertility, including treatment with the NAD + precursor NMN, along with transgenic overexpression of the NAD + biosynthetic enzymes nicotinamide mononucleotide adenyltransferase 1 and 3 (NMNAT1 and NMNAT3)

In contrast, due to the competition of tumor cells for glutamine in the TME, glutamine deficiency in immune cells leads to the inhibition of mTOR activation in immune cells [23], which then weakens TCR signaling in T cells, reduces Th1/Th17 differentiation

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