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The px459 and pBluescript vectors were co-transfected in mESCs and sgRNA/CAS9-containing cells were selected with 0.001 g/l puromycin (Merck, #P8833) after 2 days

For pH-dependent growth kinetics, bacterial cells were diluted back to an initial OD of 0.05 in 7H9 medium (supplemented with ADS and 0.2% glycerol), acidified to different pH-7.2, 6.5, 5.5, and 5.0

When taken together, these two powerhouses may work synergistically to combat inflammation more effectively than either supplement alone

However, EG-Se/Pt analogues, such as EG-Se/Cu and EG-Se/Ni, did not exhibit glutathione depletion capacity or selective killing activity in our investigation, although they can effectively kill cancer cells