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Necroptosis can also be materialized as a result of activation of the signaling pathways, due to DNA damage, which leads to the release of apoptosis-related factors from the mitochondrial intermembrane space, followed by the activation of the DNA damagerelated enzyme poly(ADP-ribose) polymerase (PARP) [213]

A549 cells were cultured in RPMI 1640 (10% FBS, 2 mM L-glutamine, 100 U/ml penicillin, and 100 g/ml streptomycin) at 37C in an atmosphere of 5% CO 2

Membrane proteins preparations incorporated into such micelles are well-suited for fluorescence-based assays due to their small, uniform particle size and significantly reduced light scattering

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