gsh glo glutathione assay promega GSH-Glo™ Did someone use a protocol

10.1016/j.redox.2012.11.008 Redox

J Environ Qual 4:473476 Ruiz-Huerta EA, Armienta-Hernndez MA, Dubrovsky JG, Gmez-Bernal JM (2022) Bioaccumulation of heavy metals and As in maize ( Zea mays L) grown close to mine tailings strongly impacts plant development

Gpxs exist in multiple isoforms, with different cellular localization and different substrate specificity ( 2 O 2 , Prxs seem to be more active, given their higher intracellular concentration and rate constant ( GSH, exploiting the redox-active thiol residue (-SH) of cysteine, exerts its antioxidant function mainly via Gpxs-mediated reactions, which result in peroxide buffering with simultaneous oxidation of GSH to GSSG ( Besides the neutralization of free radicals produced in phase 1 liver metabolism of chemical toxins, GSH also participates in the protection from the resulting electrophilic substrates through the intervention of glutathione-S-transferases (GSTs)

The toxicity can be explained by the tendency of these molecules to bind or destroy important biomolecules