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An expansion of Simons integrated hypothesis of trigger point formation

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The protein blots were incubated with first antibody for 2 h at room temperature, then probed with IRDye infrared secondary mouse or rabbit anti-rabbit IgG and visualized using the odyssey infrared imaging system (LI-COR Biosciences, Lincoln, NE, USA)

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It has been found that dysregulated keratinocytes, which include IL-1 and IL-18, are responsible for the release of AMP and IL-1 family cytokines, which then leads to the development of Th1 and Th17 cells (Anderson et al

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