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As seen in Fig 6B, over the same reference, in nonstarved cells and at 4 h of starvation, the expression of those genes was not affected by miR-4488 overexpression

142 Ghorani V, Boskabady M, Boskabady MH

After 8 h, cells were harvested in Passive Lysis Buffer (Promega Corp., Madison, WI) and the luciferase activity was measured by the Nano Dual-luciferase report assay System (Promega Corp., Madison, WI)