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The first and final ten bases of the non-contaminated reads were trimmed using DADA2 1.10.1 (ref

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You may need a supplement if you: Eat a vegetarian or vegan diet and dont consume enough foods that contain the vitamins Take heartburn medications Have had weight loss surgery Have a disease that affects your stomach lining or gut, such as IBD or Crohns disease Have or are at risk of getting age-related macular degeneration (AMD) Are older and dont get enough B vitamins through your diet In many cases, you may not need a B complex supplement, but a single B vitamin supplement

In addition to the symptoms above, B12 deficiency may lead to the following: Muscle cramps and muscle weakness : B12 deficiency negatively impacts motor and sensory nerve function, which can cause muscle cramps and weakness (7,27)