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was coupled to a timsTOF Pro 2 mass spectrometer (Bruker, Billerica, MA, USA) equipped with a CaptiveSpray ion source, operated in data-dependent acquisition with parallel accumulationserial fragmentation (DDA-PASEF) mode and dynamic exclusion

Graphical abstract Similar content being viewed by others Introduction Age-related macular degeneration (AMD) is a leading cause of vision loss globally, affecting ~ 150 million people in 2020, with that number expected to increase by nearly 50% by 2040 [1, 2]