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Protein extraction and western blotting For protein isolation, hDFs, iPSCs and MNPs were washed twice with ice-cold 1X phosphate saline buffer (1X PBS) and lysed with an extraction buffer containing 50 mM HEPES (Thermo Fisher Scientific), 150 mM NaCl (Applichem), 1 mM EDTA (pH8) (Applichem), 1 mM EGTA(pH8), 10% glycerol (Romil Pure Chemistry), and 1% Triton-X-100 (Applichem) complemented with protease (Sigma-Aldrich) and phosphatase inhibitors (PhosSTOP, Roche), according to the manufacturers protocol

An incision was made on one side of the tumor, and the skin was separated from the tumor using surgical scissors

But theres a link between oxidative stress and balding as well