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Some patients may start with more frequent treatments and then taper to a maintenance schedule, while others may require less frequent doses

coli NEBExpress(lowRF1) with 4 g ligated DNA yielded a library of 5 10 8 transformants, which was plated on four square LB/Amp agar plates (1212 cm 2 )

Mitochondrial dysfunction in COPD patients leads to the accumulation of TCA intermediates (e.g., citrate, alpha-ketoglutarate), while smoke-exposed mice exhibit mitochondrial dysfunction and reduced TCA cycle rate ( 6.3 Lipid metabolic reprogramming Normal lipid metabolism mainly consists of three core pathways: fatty acid -oxidation (FAO, a catabolic process), fatty acid synthesis, and cholesterol synthesis ( 6.3.1 Macrophage phenotypic polarization in lipid metabolism of COPD Macrophages are core cells regulating inflammation and metabolism in COPD lung tissue, and their phenotypic polarization is tightly coupled with FAO function ( The proportion of M1 macrophages is significantly increased in COPD lung tissue

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