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The supernatant was thoroughly washed in 10 mM K 2 HPO 4 and incubated in an assay buffer (1 ml): 100 mM TrisHCl (pH 8.0), 10 mM MgCl 2 , 1 mM NADP-sodium salt, 10 mM EDTA, 4.0 mM L-malate

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Second, consistent with our goal of obtaining a broader representation of laboratories that could potentially qualify as an applicable laboratory and report data, we also amended the definition of applicable laboratory to include hospital outreach laboratories that bill Medicare Part B using the CMS-1450 14x Type of Bill