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Subsequently, the ejaculates of Holstein bulls were pooled, split into four equal groups, and diluted with a Tris extender buffer supplemented with basic extender (negative control group, NC group), 2 g/ml SeNPs (SeNPs group), 4 mM GSH (GSH group), and 4 mM GSH plus 2 g/ml SeNPs (GSH + SeNPs group)

You attached a his-tag onto your protein of interest while cloning it to aid in purification, and you express your protein in Escherichia coli

mansoni by molecular typing based on the mitochondrial cytochrome c oxidase subunit 1 ( cox 1) gene according to the method detailed in Kuchta et al

Have you recently been diagnosed with a Vitamin B12 deficiency, and are unsure where to start