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But the second step in Class II involves a nucleophilic attack by the 3'-OH of the A76 ribose on the carboxyl carbon of the aminoacyl adenylate intermediate, forming aminoacyl-tRNA and AMP

and (iv) loss of function mutations in PTEN , encoding the phosphatase PTEN which inhibits PI3K

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The EdU assay demonstrated a reduction in cell proliferation following AngII exposure, whereas the AngII+siFasn group showed enhanced proliferation compared to the AngII group ( Figures 6G, H )